Antigenic relationship between mugwort and ragweed pollens by crossed immunoelectrophoresis

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Antigenic relationship between mugwort and ragweed pollens by crossed immunoelectrophoresis.

Mugwort and ragweed pollens have been considered as important respiratory allergens in Korea. These two pollens are abundant in the air of Seoul from August through October. Many ragweed-sensitive patients have shown concurrent sensitivities to mugwort pollen. However the antigenic relationship between these two pollens has not been clarified. To observe the cross-reactivity between them, we de...

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Thirty-eight of forty patients, allergic to the pollen of dwarf and giant ragweed, were found to be allergic also to the pollen of botanically related species. There was definite variation in the degree of reactivity to the various pollens in different patients. One additional patient reacted to dwarf ragweed but not to giant ragweed or to four other composites. Antibody neutralization studies ...

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Crossed immunoelectrophoresis of human platelet membranes.

Human platelet membranes were prepared by the glycerol-lysis technique of Barber and Jamieson (Barber, A. J., and Jamieson, G. A. (1970) J. Biol. Chem 245, 6357-6365), solubilized in 1% Triton X-100 and subjected to crossed immunoelectrophoresis, in 1% agarose, employing a rabbit anti-human platelet membrane antibody in the second dimension. Approximately 20 immunoprecipitates (numbered 1 to ...

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ژورنال

عنوان ژورنال: Journal of Korean Medical Science

سال: 1994

ISSN: 1011-8934,1598-6357

DOI: 10.3346/jkms.1994.9.3.213